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Creasensor: SIMPLE technology for creatinine detection in plasma.

Francesco Dal Dosso1, Deborah Decrop1, Elena Pérez-Ruiz1

  • 1BIOSYST-MeBioS, KU Leuven, Leuven, Belgium.

Analytica Chimica Acta
|January 1, 2018
PubMed
Summary
This summary is machine-generated.

The SIMPLE lab-on-a-chip platform, a self-powered microfluidic device, was successfully integrated with a creatinine assay for point-of-care diagnostics. This innovation enables rapid, cost-effective chronic kidney disease biomarker detection in plasma samples.

Keywords:
Chronic kidney diseaseCreatinineLab-on-a-chipPoint-of-careSelf-powered microfluidic pump

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Point-of-Care Diagnostics

Background:

  • Lab-on-a-chip (LOC) technologies face challenges in integrating pumping, detection, and biological assays for point-of-care (POC) applications.
  • The SIMPLE platform offers a self-powered, autonomous, cost-effective, and mass-producible solution for POC diagnostics.
  • Previous SIMPLE technology lacked demonstrated integration with complex biological assays.

Purpose of the Study:

  • To enhance the SIMPLE platform's design and fabrication for biological assay integration.
  • To demonstrate the SIMPLE platform's capability in quantifying creatinine, a biomarker for chronic kidney disease, in plasma samples.
  • To validate the robustness and performance of the SIMPLE-CREASENSOR system in an on-field setting.

Main Methods:

  • Improved SIMPLE microfluidic cartridge design and fabrication.
  • Integration of the SIMPLE cartridge with a colorimetric readout system and the benchtop Creasensor.
  • On-field validation using a single-blind study with 16 plasma samples, comparing SIMPLE-measured creatinine levels with spiked concentrations.

Main Results:

  • Successful integration of a creatinine assay onto the SIMPLE platform, enabling quantification in plasma.
  • Excellent agreement between measured and spiked creatinine concentrations (ICC: 0.97) in the on-field validation study.
  • Clinically relevant performance metrics including concentration range (0.76-20 mg/dL), low sample volume (5 μL), and rapid time-to-result (5 min).

Conclusions:

  • The SIMPLE platform demonstrates outstanding robustness for implementing biological assays, overcoming previous integration challenges.
  • The validated SIMPLE-CREASENSOR system offers a reliable and efficient POC diagnostic tool for chronic kidney disease.
  • The platform's flexibility and compatibility open avenues for diverse POC applications and more complex assays.