Chemometric-assisted method development in hydrophilic interaction liquid chromatography: A review
Maryam Taraji1, Paul R Haddad1, Ruth I J Amos1
1Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences-Chemistry, University of Tasmania, Private Bag 75, Hobart 7001, Australia.
Analytica Chimica Acta
|January 1, 2018
Summary
Computer-aided modeling, particularly quantitative structure retention relationship (QSRR) methods, significantly advances hydrophilic interaction liquid chromatography (HILIC) method development. These tools optimize HILIC separations, reducing experimental time and costs for analyzing polar compounds.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Hydrophilic interaction liquid chromatography (HILIC) is increasingly used for small polar compound determination.
- HILIC utilizes hydro-organic mobile phases and hydrophilic stationary phases.
Purpose of the Study:
- To review the application of computer-aided modeling in HILIC.
- To highlight the role of quantitative structure retention relationship (QSRR) in HILIC method development.
Main Methods:
- Focus on computer-aided modeling tools for HILIC.
- Application of quantitative structure retention relationship (QSRR) modeling.
- Integration with experimental design philosophy.
Main Results:
- Computer-aided modeling aids in understanding HILIC retention mechanisms.
- These tools assist in classifying HILIC stationary phases.
- Prediction of retention times and optimization of chromatographic conditions are facilitated.
Conclusions:
- Robust QSRR models enhance HILIC applications across diverse research areas.
- Predictive modeling minimizes experimental costs and time.
- QSRR combined with experimental design optimizes HILIC method development.
Keywords:
ChemometricsHydrophilic interaction liquid chromatographyMethod developmentQuantitative structure-retention relationshipsMore Related Videos
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