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Updated: Feb 16, 2026

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins
Published on: March 11, 2020
Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite
S Meyer1, A López-Serrano2, H Mitze3
1Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. tanja.schwerdtle@uni-potsdam.de and NutriAct - Competence Cluster Nutrition Research Berlin-Potsdam, Germany.
Abstract:
Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.
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