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Related Experiment Video

Updated: Feb 16, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
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Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index

Shwetadwip Chowdhury1, Will J Eldridge1, Adam Wax1

  • 1Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, 1427 FCIEMAS, 101 Science Drive Box 90281, Durham, North Carolina 27708, USA.

Biomedical Optics Express
|January 4, 2018
PubMed
Summary

Structured illumination microscopy (SI) now achieves 3D dual-modality imaging, combining super-resolution fluorescence and refractive-index visualization for biological samples.

Keywords:
(110.6960) Tomography(180.6900) Three-dimensional microscopy

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Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Microscopy

Background:

  • Structured illumination (SI) microscopy is traditionally used for fluorescent super-resolution imaging.
  • Emerging research indicates SI's potential for sub-diffraction resolution coherent imaging with endogenous contrast.

Purpose of the Study:

  • To demonstrate a novel SI microscopy framework integrating fluorescent super-resolution and coherent synthetic aperture principles.
  • To achieve simultaneous 3D sub-diffraction resolution fluorescence and refractive-index (RI) visualization of biological specimens.

Main Methods:

  • Development and experimental implementation of a SI microscope.
  • Integration of fluorescent super-resolution and coherent synthetic aperture techniques.
  • 3D reconstruction of fluorescence and RI data.

Main Results:

  • Successful demonstration of a 3D dual-modality SI microscope.
  • Experimental reconstruction of 3D RI/fluorescence visualizations for calibration microspheres and A549/HT-29 cells.
  • Achieved sub-diffraction resolution for both fluorescence and RI imaging modalities.

Conclusions:

  • SI microscopy can be effectively adapted for dual-modality, sub-diffraction resolution imaging.
  • This integrated approach offers a promising platform for visualizing synergistic biophysical/biochemical and molecular mechanisms.
  • Potential for advancing biological studies requiring simultaneous multi-modal sub-diffraction resolution imaging.