Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Balancing Redox Equations02:58

Balancing Redox Equations

62.7K
Electrochemistry is the science involved in the interconversion of electrical and chemical reactions. Such reactions are called reduction-oxidation, or redox reactions. These important reactions are defined by changes in oxidation states for one or more reactant elements and include a subset of reactions involving the transfer of electrons between reactant species. Electrochemistry as a field has evolved to yield sufficient insights on the fundamental principles of redox chemistry and multiple...
62.7K
Redox Reactions01:24

Redox Reactions

59.0K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
59.0K
Redox Reactions01:27

Redox Reactions

1.2K
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
1.2K
Redox Equilibria: Overview01:23

Redox Equilibria: Overview

1.6K
A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
1.6K
Redox Titration: Overview01:21

Redox Titration: Overview

5.1K
Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...
5.1K
Ladder Diagrams: Redox Equilibria01:30

Ladder Diagrams: Redox Equilibria

809
Ladder diagrams are useful tools for understanding redox equilibrium reactions, especially the effects of concentration changes on the electrochemical potential of the reaction. The vertical axis in the redox ladder diagrams represents the electrochemical potential, E. The area of predominance is demarcated using the Nernst equation.
Consider the Fe3+/Fe2+ half-reaction, which has a standard-state potential of +0.771 V. At potentials more positive than +0.771 V, Fe3+ predominates, whereas Fe2+...
809

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical outcomes in abdominal wall autologous flap reconstruction: a systematic review.

Hernia : the journal of hernias and abdominal wall surgery·2026
Same author

Safety Assessment of Radish Root - Derived Ingredients as Used in Cosmetics.

International journal of toxicology·2026
Same author

Amended Safety Assessment of Naturally-Sourced Clays as Used in Cosmetics.

International journal of toxicology·2026
Same author

Safety Assessment of Diatomaceous Earth as Used in Cosmetics.

International journal of toxicology·2026
Same author

Safety Assessment of Basic Yellow 87 as Used in Cosmetics.

International journal of toxicology·2026
Same author

Safety Assessment of Glycolactones as Used in Cosmetics.

International journal of toxicology·2026

Related Experiment Video

Updated: Feb 16, 2026

Cellular Redox Profiling Using High-content Microscopy
11:37

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

11.6K

Redox modulation of NQO1.

David Siegel1, Donna D Dehn1, Samantha S Bokatzian2

  • 1Department of Pharmaceutical Sciences, Skaggs School of Pharmacy, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States of America.

Plos One
|January 4, 2018
PubMed
Summary

The enzyme NAD(P)H:quinone oxidoreductase 1 (NQO1) changes structure based on cellular redox state. This structural change affects its interactions, potentially regulating cellular processes like microtubule deacetylation.

More Related Videos

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
06:01

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

13.9K
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
12:07

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

16.8K

Related Experiment Videos

Last Updated: Feb 16, 2026

Cellular Redox Profiling Using High-content Microscopy
11:37

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

11.6K
EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
06:01

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

13.9K
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
12:07

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

16.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • NAD(P)H:quinone oxidoreductase 1 (NQO1) is a key enzyme in cellular redox homeostasis.
  • Cellular interactions of NQO1 may be modulated by pyridine nucleotide concentrations.
  • NAD(P)H binding is suggested to alter NQO1's structure and stability.

Purpose of the Study:

  • To investigate the structural changes in NQO1 induced by NAD(P)H binding.
  • To determine if NQO1's immunoreactivity reflects the cellular NAD(P)+/NAD(P)H ratio.
  • To explore the potential role of NQO1 in microtubule deacetylation during cell division.

Main Methods:

  • Purification of NQO1 and in vitro studies of NAD(P)H-induced structural changes.
  • Immunoprecipitation assays to assess NQO1's binding to antibodies under varying redox conditions.
  • Immunostaining in human cells to visualize NQO1 localization and its correlation with cellular redox state and microtubule acetylation.

Main Results:

  • NAD(P)H addition to purified NQO1 caused a conformational change, reducing antibody binding to specific domains.
  • Treatment with β-lapachone, which oxidizes NAD(P)H, increased NQO1's immunoreactivity and facilitated its immunoprecipitation.
  • Immunostaining revealed NQO1 co-localization with acetylated α-tubulin and Sirt2 at the centrosome and mitotic spindle, suggesting a role in microtubule deacetylation.

Conclusions:

  • NQO1's structure and immunoreactivity are sensitive to the cellular NAD(P)+/NAD(P)H redox ratio.
  • NQO1 may function as a redox-dependent molecular switch, altering its interactions based on the cellular environment.
  • NQO1 potentially supplies NAD+ for Sirt2-mediated microtubule deacetylation during the cell cycle.