Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

48.9K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
48.9K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

53.2K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
53.2K
Labeling DNA Probes03:31

Labeling DNA Probes

9.5K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.5K
Genomics02:02

Genomics

40.9K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

37.3K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.3K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

9.2K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Micro-comb 3D printing: rapid fabrication of tissue-guiding substrates using micro-embossed nozzles.

Biofabrication·2026
Same author

Single-Molecule Imaging and Spectroscopy Enables Quantification of Location-Dependent Light-Matter Interactions on Nanoantennas.

Small science·2026
Same author

Liposome purification from micromolar protein background using diffusiophoretic trapping.

Nanoscale·2026
Same author

Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence.

Nanophotonics (Berlin, Germany)·2025
Same author

In-Solution Characterization of Biomolecular Interaction Kinetics under Native Conditions.

Analytical chemistry·2025
Same author

Effects of Ultrasound Treatment on Emulsifying Properties of Pea Protein Isolates Obtained from Four Different Pea Flour Varieties.

Foods (Basel, Switzerland)·2025

Related Experiment Video

Updated: Feb 16, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

20.5K

Concentrating and labeling genomic DNA in a nanofluidic array.

Rodolphe Marie1, Jonas N Pedersen, Kalim U Mir

  • 1Department of Micro and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmark. rcwm@nanotech.dtu.dk.

Nanoscale
|January 5, 2018
PubMed
Summary

This study introduces a novel method for DNA sequencing-by-synthesis using entropic trapping to arrange single DNA molecules without surface immobilization. This approach enhances enzymatic processing for genomic DNA analysis, particularly for single-cell applications.

More Related Videos

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

13.3K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.1K

Related Experiment Videos

Last Updated: Feb 16, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

20.5K
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

13.3K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.1K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • DNA sequencing-by-synthesis relies on nucleotide incorporation by DNA polymerase.
  • Current methods immobilize DNA or enzymes on surfaces, potentially limiting polymerase activity.

Purpose of the Study:

  • To develop a device and protocol for enzymatic labeling of genomic DNA without surface attachment.
  • To overcome limitations of solid-phase reactions in DNA polymerase applications.

Main Methods:

  • Utilizing a device with a dense array of pits within a nanoslit for entropic trapping of DNA molecules.
  • Performing enzymatic labeling via ϕ29 polymerase extension from single-strand nicks on trapped DNA.
  • Achieving high DNA molecule density and concentration through entropic forces.

Main Results:

  • Demonstrated efficient loading of DNA molecules into the entropic trap array (>90% capacity).
  • Achieved high DNA concentrations (up to 100 μg mL⁻¹).
  • Showcased successful enzymatic processing (nucleotide incorporation) on surface-free DNA.

Conclusions:

  • The developed device enables surface-free enzymatic processing of genomic DNA.
  • This method is suitable for preparing DNA from single cells for microfluidic applications.
  • The entropic trapping approach offers a promising alternative for DNA sequencing and analysis.