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Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo
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Protein Determination-Method Matters.

Hanne K Mæhre1, Lars Dalheim2, Guro K Edvinsen3

  • 1Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UIT The Arctic University of Norway, N-9037 Tromsø, Norway. hanne.maehre@uit.no.

Foods (Basel, Switzerland)
|January 6, 2018
PubMed
Summary
This summary is machine-generated.

Comparing food protein analysis methods reveals most overestimate content due to indirect measurements. Amino acid analysis is recommended for accurate food protein determination, despite needing hydrolysis method improvements.

Keywords:
BradfordKjeldahlLowryamino acidsanalytical methodsextraction methodsproteins

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Area of Science:

  • Food science
  • Analytical chemistry
  • Biochemistry

Background:

  • Protein content determination in foods varies significantly by analytical method.
  • Direct comparison of protein analysis results across studies is challenging due to methodological differences.

Purpose of the Study:

  • To examine and compare various protein analytical methods used in food analysis.
  • To assess the impact of extraction efficiency on protein determination accuracy.
  • To identify reliable methods for precise food protein quantification.

Main Methods:

  • Comparison of multiple protein analytical techniques.
  • Determination of protein extraction yields across different food matrices.
  • Evaluation of indirect measurement methods (e.g., nitrogen determination) versus direct methods.

Main Results:

  • Most analytical methods overestimated food protein content.
  • Inaccuracies were primarily linked to indirect nitrogen determination and conversion to protein.
  • Interference from other chemical substances in the food matrix affected results.

Conclusions:

  • Amino acid analysis is the most accurate protein determination method, unaffected by interfering substances.
  • While hydrolysis requires optimization, it is the recommended method for food protein analysis.
  • Standardizing protein analysis methods is crucial for reliable food composition data.