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Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform

Maria Guarnaccia1, Rosario Iemmolo2, Floriana San Biagio3

  • 1Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami 18, 95126 Catania, Italy. maria.guarnaccia@cnr.it.

Sensors (Basel, Switzerland)
|January 6, 2018
PubMed

Insights

A new Lab-on-Chip assay simplifies KRAS genotyping for colorectal cancer (CRC) patients. This rapid, accurate method aids personalized treatment by detecting key KRAS mutations, improving clinical routine diagnostics.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biotechnology

Background:

  • KRAS mutations drive colorectal cancer (CRC) pathogenesis, correlating with poor prognosis and resistance to anti-EGFR therapies.
  • KRAS mutation testing is crucial for personalized CRC treatment, but current methods are costly, slow, and lack sensitivity.
  • There is a need for efficient, accurate KRAS mutation detection in clinical settings.

Purpose of the Study:

  • To develop and validate a novel Lab-on-Chip assay for KRAS genotyping in colorectal cancer.
  • To create an integrated sample-to-result system for rapid and accurate detection of KRAS mutations.
  • To assess the potential of this platform for routine clinical use.

Main Methods:

  • Development of a Lab-on-Chip assay utilizing the In-Check platform.
  • Integration of microfluidic handling, multiplex polymerase chain reaction (PCR), and low-density microarray.
  • Detection of KRAS point mutations across multiple codons (12, 13, 59, 61, 117, 146) in exons 2, 3, and 4.

Main Results:

  • The Lab-on-Chip assay successfully integrated microfluidics, multiplex PCR, and microarray for KRAS genotyping.
  • The system demonstrated the ability to detect KRAS mutations in codons 12, 13, 59, 61, 117, and 146.
  • The assay offers miniaturization, automation, rapid analysis, and high accuracy and reproducibility.

Conclusions:

  • The developed Lab-on-Chip assay provides a simplified and efficient method for KRAS genotyping.
  • This platform has the potential to significantly improve the clinical routine for personalized CRC treatment.
  • The assay's advantages include speed, accuracy, and reproducibility, addressing limitations of current detection methods.
Keywords:
KRASLab-on-ChipMicroarrayPCRdiagnosticnucleic acids analysis

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