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Published on: November 9, 2014
Modulation of gingival cell response towards dental composites
A Jerg1, S D Schulz2, P Tomakidi2
1Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg i. Br., Germany; Department of Oral Biotechnology, Center of Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg i. Br., Germany.
Dental composite eluates impact human gingival fibroblasts and keratinocytes. Self-adhering composites showed more significant cell behavior modulation, affecting proliferation, adhesion, and inflammation markers.
Area of Science:
- Biomaterials Science
- Cell Biology
- Dental Materials
Background:
- Dental composites are widely used in restorative dentistry.
- Understanding their biocompatibility with oral cells is crucial for clinical success.
- Eluates from dental composites can influence cellular behavior.
Purpose of the Study:
- To investigate the cellular response of human gingival fibroblasts (HGF) and keratinocytes (HGK) to eluates from clinically applied dental composites.
- To evaluate the impact of different dental composite materials on cell adhesion, proliferation, and inflammatory responses.
Main Methods:
- Exposure of HGF and HGK to eluates from five dental composites (Ceram X™, Filtek™ Silorane, Filtek™ Supreme XTE, Fusio™ Liquid Dentin, Vertise™ Flow) stored for 24h and 72h.
- Cellular responses assessed via real-time impedance analysis for adhesion/proliferation, indirect immunofluorescence for biomarkers, and ELISA for IL-6 secretion.
Main Results:
- Eluates generally induced gradual cellular responses in both HGF and HGK.
- Fusio™ Liquid Dentin and Vertise™ Flow eluates activated ERK 1/2 in HGK and reduced proliferation/adhesion.
- Exposure to Filtek™ Supreme XTE, Fusio™ Liquid Dentin, and Vertise™ Flow eluates decreased IL-6 secretion in HGK after 72h.
Conclusions:
- All tested dental composites modulated cellular behavior, with self-adhering materials showing a more pronounced effect.
- Cellular responses involve modulation of signal transduction, differentiation, and inflammation pathways.
- Findings highlight the importance of considering material-specific cellular interactions in dental applications.
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