Related Experiment Video
Updated: Feb 16, 2026
09:19
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
Published on: August 29, 2025
638
A bio-image sensor for simultaneous detection of multi-neurotransmitters
You-Na Lee1, Koichi Okumura1, Tomoko Horio1
1Electrical & Electronic Information Eng., Toyohashi University of Technology, Hibarigaoka 1-1, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
Talanta
|January 10, 2018
Summary
This study introduces a novel bio-image sensor for real-time, simultaneous detection of neurotransmitters like adenosine 5'-triphosphate (ATP) and acetylcholine (ACh). It features enhanced spatial resolution to minimize signal overlap, enabling precise neurochemical monitoring.
Area of Science:
- Biosensors and Bioelectronics
- Neuroscience and Neurochemistry
- Chemical Sensing and Imaging
Background:
- Simultaneous detection of multiple neurotransmitters is crucial for understanding complex neural processes.
- Existing methods often face challenges with spatial resolution and signal interference, particularly from hydrogen ion (H+) diffusion.
- Real-time monitoring of neurotransmitter dynamics requires advanced sensing technologies.
Purpose of the Study:
- To develop and characterize a novel bio-image sensor for simultaneous spatial and temporal detection of neurotransmitters.
- To improve spatial resolution by mitigating hydrogen ion (H+) diffusion interference.
- To demonstrate the sensor's capability for real-time imaging of neurotransmitter concentration gradients.
Main Methods:
- Fabrication of a 128 × 128 pixel array bio-image sensor with enzyme-immobilized membranes.
- Immobilization of apyrase and acetylcholinesterase (AChE) for selective detection of adenosine 5 extprime-triphosphate (ATP) and acetylcholine (ACh).
- Integration of hydrogen ion (H+) diffusion barrier layers to enhance spatial resolution and minimize signal overlap.
- Experimental determination of sensing characteristics, including sensitivity and limit of detection.
Main Results:
- Successful development of a bio-image sensor capable of simultaneous multi-neurotransmitter detection.
- Demonstration of effective H+ diffusion barrier layers for improved spatial resolution.
- Achieved H+ diffusion-independent, real-time imaging of ATP and ACh concentration gradients.
- Characterization of the sensor's sensitivity and limit of detection.
Conclusions:
- The proposed bio-image sensor offers a robust platform for high-resolution, real-time monitoring of neurotransmitter activity.
- The H+ diffusion barrier technology effectively addresses signal overlap issues, enhancing spatial accuracy.
- This technology holds potential for customizable monitoring of various neurochemicals by employing different enzyme-based selective elements.
Keywords:
Bio-image sensorEnzyme-immobilized membraneH(+) diffusion-barrier layerMulti neurotransmittersMultiplexing bio-image sensorSimultaneous detectionMore Related Videos
Related Concept Videos
01:31Neurotransmitters
2.9K
Neurotransmitters are essential chemical messengers within the nervous system, facilitating the communication between neurons. These chemical messengers, varying in function and effect, are critical for sustaining various aspects of neurological health and emotional well-being.
2.9K
01:30Neurotransmitters
5.2K
Neurotransmitters play a crucial role in the communication between neurons in the autonomic nervous system. Neurons in the autonomic nervous system can be cholinergic or adrenergic depending on the neurotransmitters synthesized. Cholinergic neurons use acetylcholine as their primary neurotransmitter. This includes all the preganglionic fibers of the sympathetic and pre- and postganglionic fibers of the parasympathetic nervous systems. In addition, neurons of the somatic nervous system also use...
5.2K
01:30Classification of Neurotransmitters
5.4K
Neurotransmitters play a crucial role in the communication between neurons in the autonomic nervous system. Neurons in the autonomic nervous system can be cholinergic or adrenergic depending on the neurotransmitters synthesized. Cholinergic neurons use acetylcholine as their primary neurotransmitter. This includes all the preganglionic fibers of the sympathetic and pre- and postganglionic fibers of the parasympathetic nervous systems. In addition, neurons of the somatic nervous system also use...
5.4K
01:23Role of Neurotransmitters in Memory
2.6K
Neurotransmitters are integral to the brain's communication system, enabling neurons to transmit signals across synapses. This chemical exchange underpins various cognitive functions, including memory processes. The role of neurotransmitters in memory is multifaceted, influencing the encoding, consolidation, and retrieval of memories through their action on different neural circuits.
Glutamate and Synaptic Plasticity
Glutamate, the brain's main excitatory neurotransmitter, is...
Glutamate and Synaptic Plasticity
Glutamate, the brain's main excitatory neurotransmitter, is...
2.6K
01:29Drugs Affecting Neurotransmitter Synthesis
2.2K
Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase,...
2.2K
01:29Excitatory and Inhibitory Effects of Neurotransmitters
13.4K
When an action potential reaches the presynaptic axon terminal, it releases neurotransmitters from the neuron into the synaptic cleft at a chemical synapse. The released neurotransmitter can be excitatory or inhibitory. The critical criteria commonly used to determine whether a molecule is a neurotransmitter at a chemical synapse are the molecule's presence in the presynaptic neuron. Second, its release is in response to strong presynaptic depolarization. And lastly, the presence of...
13.4K