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Investigating cell-type specific functions of tenascin-C

Devadarssen Murdamoothoo1, Anja Schwenzer2, Jessica Kant1

  • 1Inserm U1109, The Microenvironmental Niche in Tumorigenesis and Targeted Therapy (MNT3) Team, Strasbourg, France; Université de Strasbourg, LabEx Medalis, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.

Methods in Cell Biology
|January 10, 2018
PubMed

Insights

Tenascin-C (TNC) is crucial in tissue repair, immunity, and angiogenesis. Assays detailed here explore TNC's effects on cell behavior and its role in various diseases, aiding further research.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Tenascin-C (TNC) is an extracellular matrix protein with significant roles in tissue repair, inflammation, fibrosis, and cancer.
  • Mouse models have confirmed TNC's importance in immunity and angiogenesis.
  • Existing research utilizes cell and organ cultures to understand TNC's in vivo mechanisms.

Purpose of the Study:

  • To describe assays investigating tenascin-C's (TNC) antiadhesive properties.
  • To measure TNC's effects on cell cytoskeleton, survival, proliferation, and migration.
  • To assess TNC's impact on endothelial and immune cells and compare fibroblast responses.

Main Methods:

  • Investigating TNC's antiadhesive properties through specific assays.
  • Measuring TNC's influence on cellular processes like actin cytoskeleton dynamics, survival, proliferation, and migration.
  • Utilizing cell and organ cultures to study TNC's effects on endothelial and immune cells.
  • Comparing responses of fibroblasts from normal and diseased tissues to TNC.

Main Results:

  • Assays were developed to quantify TNC's impact on cell behavior.
  • TNC's role in modulating endothelial and immune cell function was investigated.
  • Differential responses of fibroblasts from healthy versus diseased tissues were observed.

Conclusions:

  • The described assays provide valuable tools for studying tenascin-C's functions.
  • Understanding TNC's cellular mechanisms is critical for its role in disease.
  • Further research can leverage these methods to explore TNC in various pathological contexts.
Keywords:
Actin cytoskeletonCell adhesionChicken chorioallantoic membrane assayEndothelial cellImmune cell activationImmunofluorescenceMatrigel tubulogenesis assayProliferation assaySurvival assaySynovial fibroblastTenascin-CWound healing assayextracellular matrix

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