Quantitative Analysis of Cellular Proteome Alterations in CDV-Infected Mink Lung Epithelial Cells

Area of Science:

• Virology
• Cell Biology
• Proteomics

Background:

• Canine distemper virus (CDV) is a lethal paramyxovirus affecting carnivores, including mink.
• Mink lung epithelial cells (Mv.1.Lu) serve as a relevant model for studying CDV infection.
• Understanding cellular responses to CDV is crucial for disease management.

Purpose of the Study:

• To comprehensively analyze the proteomic response of Mv.1.Lu cells to CDV infection.
• To identify differentially expressed proteins (DEPs) and associated signaling pathways.
• To elucidate molecular mechanisms underlying CDV pathogenesis and immune response.

Main Methods:

• iTRAQ combined with LC-MS/MS for proteomic profiling.
• Real-time RT-PCR and western blot for validating DEPs.
• Network and KEGG pathway analysis to identify regulated pathways.
• Western blot and immunofluorescence assays to confirm NF-κB activation.

Main Results:

• Identified 151 up-regulated and 369 down-regulated proteins in CDV-infected Mv.1.Lu cells.
• Confirmed NF-κB signaling pathway as significantly affected by CDV infection.
• Demonstrated CDV-induced phosphorylation and nuclear translocation of NF-κB P65.
• Identified potential DEPs involved in CDV pathogenesis and immune response.

Conclusions:

• This study provides the first proteomic overview of Mv.1.Lu cell response to CDV.
• CDV infection activates the NF-κB signaling pathway in mink lung cells.
• Findings offer insights into molecular mechanisms of CDV pathogenesis and host immunity.