Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

3.9K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
3.9K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

989
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
989
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.3K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

1.5K
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
1.5K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.6K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolotheranostics of pancreatic cancer by targeting choline, glutamine, and glucose transporters with photoimmunotherapy.

Npj imaging·2026
Same author

Human epidermal growth factor receptor 3-targeted near-infrared photoimmunotherapy in a xenograft mouse model of breast cancer.

BMC cancer·2026
Same author

Epidermal growth factor receptor-targeted near-infrared photoimmunotherapy for malignant pleural mesothelioma.

Lung cancer (Amsterdam, Netherlands)·2026
Same author

Understanding tumor microenvironment dynamics and immune checkpoint inhibitor efficacy following mEGFR-targeted near-infrared photoimmunotherapy.

Cancer letters·2026
Same author

Near-infrared Photoimmunotherapy Targeting High-risk Human Neuroblastoma Cells Expressing GD2.

Anticancer research·2025
Same author

Enhancement of drug delivery through fibroblast activation protein-targeted near-infrared photoimmunotherapy.

JCI insight·2025
Same journal

Artificial intelligence for biomarker prediction in gastric cancer: from histopathology to multimodal integration.

Frontiers in oncology·2026
Same journal

Influencing factors of cognitive frailty in older patients with esophageal cancer undergoing chemotherapy: a mixed-methods study.

Frontiers in oncology·2026
Same journal

HDAC1-mediated CDK1 decrotonylation inhibits colorectal cancer proliferation by regulating cell cycle and apoptosis.

Frontiers in oncology·2026
Same journal

Development and validation of a machine learning model to evaluate survival in patients with newly diagnosed breast cancer with liver metastasis.

Frontiers in oncology·2026
Same journal

Primary diffuse large b-cell lymphoma of the lip: a case report and literature review.

Frontiers in oncology·2026
Same journal

A deep learning-based prognostic model for diffuse large B-cell lymphoma incorporating PET/CT imaging features.

Frontiers in oncology·2026
See all related articles

Related Experiment Video

Updated: Feb 15, 2026

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery
11:05

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

Published on: September 19, 2014

12.7K

Fluorescence-Guided Surgery.

Tadanobu Nagaya1, Yu A Nakamura1, Peter L Choyke1

  • 1Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.

Frontiers in Oncology
|January 10, 2018
PubMed
Summary
This summary is machine-generated.

Fluorescence-guided surgery (FGS) enhances cancer resection by improving tumor visualization. New fluorescent probes aid surgeons in precisely locating tumors and margins, leading to safer, more complete cancer removal.

Keywords:
activatable probealways-on probefluorescence-guided surgerymolecular imagingmonoclonal antibodies

More Related Videos

Survivable Stereotaxic Surgery in Rodents
00:08

Survivable Stereotaxic Surgery in Rodents

Published on: October 6, 2008

70.2K
Endoscopic Cholesteatoma Surgery
08:47

Endoscopic Cholesteatoma Surgery

Published on: January 19, 2022

13.3K

Related Experiment Videos

Last Updated: Feb 15, 2026

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery
11:05

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

Published on: September 19, 2014

12.7K
Survivable Stereotaxic Surgery in Rodents
00:08

Survivable Stereotaxic Surgery in Rodents

Published on: October 6, 2008

70.2K
Endoscopic Cholesteatoma Surgery
08:47

Endoscopic Cholesteatoma Surgery

Published on: January 19, 2022

13.3K

Area of Science:

  • Oncology
  • Surgical Innovation
  • Medical Imaging

Background:

  • Surgical cancer resection is a key treatment, but relies on conventional white light imaging.
  • Accurate tumor localization and margin definition during surgery remain challenging.
  • Advances in preoperative imaging do not fully overcome intraoperative localization difficulties.

Purpose of the Study:

  • To review current and novel fluorescent probes for enhancing fluorescence-guided surgery (FGS).
  • To highlight the potential of FGS in improving tumor visualization and resection accuracy.
  • To discuss how FGS can optimize surgical outcomes and patient safety.

Main Methods:

  • Literature review of existing and emerging fluorescent probes for FGS.
  • Analysis of the role of intraoperative visualization in surgical oncology.
  • Synthesis of information on probe development and application in FGS.

Main Results:

  • FGS offers improved intraoperative visualization of tumors and their margins.
  • New fluorescent probes are expanding the utility and availability of FGS.
  • FGS can lead to more complete tumor resections and reduced damage to healthy tissue.

Conclusions:

  • Fluorescence-guided surgery (FGS) represents a significant advancement in oncologic surgery.
  • The development of new fluorescent probes is crucial for the broader adoption and efficacy of FGS.
  • FGS has the potential to improve surgical safety, reduce operative time, and decrease the need for repeat surgeries.