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Published on: September 17, 2021
Alkoxy Tetrazine Substitution at a Boron Center: A Strategy for Synthesizing Highly Fluorogenic Hydrophilic Probes
Min Wu1, Xiaoai Wu2, Yayue Wang1
1Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital, West China Medical School, Sichuan University, 001 Forth Keyuan Road, 610041, Chengdu, P.R. China.
Abstract:
Strongly fluorogenic boron dipyrromethene (BODIPY)-tetrazine probes have been obtained by introducing an alkoxy tetrazine fragment at the boron center. The fluorescence signal from these probes strongly increases by up to 225-fold after reaction with bioorthogonal coupling partners, and the hydrophilicity of probes is improved, such that they are suitable for live-cell imaging.
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