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Updated: Feb 15, 2026
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Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
Published on: November 17, 2011
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Field of view scanning based quantitative interferometric microscopic cytometers for cellular imaging and analysis
Keding Yan1,2, Liang Xue3,2, Shouyu Wang4,5,2
1School of Electronic Information Engineering, Xi'an Technological University, Xi'an, Shaanxi 710032, China.
Microscopy Research and Technique
|January 10, 2018
Summary
Three new quantitative interferometric microscopic cytometers (QIMCs) enable massive cell measurements. These devices offer quantitative imaging and large-amount sampling for biological and medical applications.
Area of Science:
- Biomedical Engineering
- Optical Microscopy
- Cell Biology
Background:
- Traditional microscopy provides limited qualitative data and fixed fields of view (FoV).
- Quantitative cellular measurements are crucial for disease diagnosis and biological research.
- Limitations in sample observation hinder large-amount cell analysis.
Purpose of the Study:
- To introduce and compare three novel quantitative interferometric microscopic cytometers (QIMCs).
- To enable quantitative measurements of massive cell populations.
- To overcome the limitations of traditional microscopy in cell analysis.
Main Methods:
- Development of three QIMCs utilizing Field of View (FoV) scanning.
- Quantitative phase distribution retrieval across an extended FoV.
- Measurement of cellular parameters including volume, area, and roundness.
Main Results:
- The designed QIMCs successfully retrieve quantitative phase distributions.
- Detailed cellular information (volume, area, roundness) is obtained for numerous cells.
- Extended FoV scanning allows for large-amount sample observations.
Conclusions:
- QIMCs provide quantitative imaging and large-amount sampling capabilities.
- These instruments are suitable for high-throughput cell imaging and statistical analysis.
- Potential applications in biological research and medical diagnostics are highlighted.
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