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Cholesterol imprinted composite membranes for selective cholesterol recognition from intestinal mimicking solution
Mehmet Odabaşı1, Lokman Uzun2, Gözde Baydemir3
1Aksaray University, Department of Chemistry, Aksaray, Turkey.
Colloids and Surfaces. B, Biointerfaces
|January 10, 2018
Summary
We developed cholesterol-specific polymer membranes using molecular imprinting. These membranes demonstrate high selectivity and capacity for cholesterol adsorption, even after multiple uses.
Area of Science:
- Materials Science
- Polymer Chemistry
- Analytical Chemistry
Background:
- Molecularly imprinted polymers (MIPs) are designed with template-specific cavities for selective adsorption.
- Developing efficient adsorbents for target molecules like cholesterol is crucial in various applications.
Purpose of the Study:
- To prepare and characterize cholesterol-imprinted poly(2-hydroxyethyl methacrylate-methacryloyamidotryptophan) (PHEMA-MTrp) particles embedded composite membranes.
- To evaluate the adsorption performance and selectivity of these membranes for cholesterol.
Main Methods:
- Synthesis of cholesterol-imprinted PHEMA-MTrp particles and their incorporation into composite membranes.
- Characterization using elemental analysis, FTIR, SEM, swelling tests, and surface area measurements.
- Batch adsorption experiments in methanol and intestinal-mimicking solutions, with selectivity tests against stigmasterol and estradiol.
Main Results:
- MIP particle-embedded membranes exhibited a higher specific surface area (36.5 m²/g) compared to NIP (29.2 m²/g) and particle-free membranes (13.7 m²/g).
- The imprinted membranes showed 1.96 and 2.13 times greater selectivity for cholesterol over stigmasterol and estradiol, respectively.
- Cholesterol adsorption capacity reached 23.43 mg/g at a concentration of 2 mg/mL, with negligible capacity loss after ten adsorption cycles.
Conclusions:
- The prepared cholesterol-imprinted composite membranes possess high selectivity and adsorption capacity.
- These membranes demonstrate excellent reusability, making them promising for cholesterol separation and purification.
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