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Updated: Feb 15, 2026

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
Published on: February 3, 2021
Competitive annealing mediated isothermal amplification of nucleic acids
Rui Mao1, Lifei Qi2, Jianjun Li3
1State Key Laboratory of Biochemical Engineering and Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, P.R. China. ygdu@ipe.ac.cn and University of Chinese Academy of Sciences, Beijing 100049, P.R. China and Zhongke Xinray (Suzhou) biological Science Technologies Co. Ltd, Suzhou 215152, P.R. China. xinray_sz@yeah.net.
Abstract:
A novel nucleic acid isothermal amplification method with high specificity, efficiency and rapidity was developed. The principle of primer design was achieved by slight modification of PCR primers. Acceleration probes were also explored. The newly developed method is of great potential for point of care diagnostics.
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