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Updated: Feb 15, 2026

Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture
Aastha Chhabra1,2, Vibha Rani3
1Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, India.
Abstract:
Gelatin zymography, first described by Heussen and Dowdle in the 1980s, is a widely used valuable tool in research and diagnostics. The technique identifies gelatinases by the degradation of their preferential substrate as well as by their molecular weight (kDa). We here describe detailed methodology for the detection of pro- and active- forms of both MMP-2 (gelatinase A) and MMP-9 (gelatinase B) in cells using norepinephrine-stimulated H9c2 cardiomyoblasts as model. An easy to follow step-by-step protocol has been carefully written for reliable results. We also suggest an acceptable method for quantification of gelatin zymograms.
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