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Cancer Susceptibility Models in Protease-Deficient Mice
Alicia R Folgueras1, Sandra Freitas-Rodríguez2, Yaiza Español2
1Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Oviedo, Spain. arfolgueras@uniovi.es.
Methods in Molecular Biology (Clifton, N.J.)
|January 11, 2018
Summary
Proteases play complex roles in cancer, acting as both promoters and suppressors of tumor growth. This chapter details methods using genetically modified mouse models to study protease functions in various cancers.
Area of Science:
- Biochemistry
- Molecular Biology
- Cancer Research
Background:
- Proteases are enzymes that degrade cellular surroundings, historically linked to promoting cancer invasion and spread.
- Recent studies using protease-deficient mouse models reveal diverse roles, including significant tumor-suppressive functions.
Purpose of the Study:
- To describe methods for chemically inducing various cancers in genetically modified mouse models.
- To enable efficient evaluation of the specific pro- or antitumoral functions of proteases in cancer.
Main Methods:
- Chemical induction of specific cancers (lung adenocarcinoma, hepatocellular carcinoma, oral and esophageal carcinoma, colorectal carcinoma, skin cancer, fibrosarcoma) in genetically modified mice.
- Utilizing these models to assess the dual roles of proteases in cancer progression and suppression.
Main Results:
- Established protocols for generating diverse cancer models in mice.
- Demonstrated the utility of these models for dissecting protease functions in cancer biology.
Conclusions:
- Genetically modified mouse models are crucial for understanding the complex roles of proteases in cancer.
- These methods allow for the precise evaluation of both pro- and antitumoral activities of proteases.
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