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Extraction of Extracellular Vesicles from Whole Tissue
Published on: February 7, 2019
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Extracellular Vesicles Provide a Means for Tissue Crosstalk during Exercise
Martin Whitham1, Benjamin L Parker2, Martin Friedrichsen3
1Cellular and Molecular Metabolism, Garvan Institute of Medical Research, Sydney, NSW, Australia; St Vincent's Clinical School, University of New South Wales, Sydney, NSW, Australia.
Cell Metabolism
|January 11, 2018
Summary
Exercise releases over 300 proteins via extracellular vesicles (EVs), particularly exosomes, into circulation. These exercise-induced EVs may transfer cargo, influencing systemic adaptations and revealing new myokine signaling pathways.
Area of Science:
- Exercise physiology
- Molecular biology
- Proteomics
Background:
- Exercise triggers inter-tissue signaling proteins crucial for adaptation.
- Circulating proteins are often packaged within extracellular vesicles (EVs).
Purpose of the Study:
- To characterize exercise-induced secretion of EV-contained proteins.
- To identify novel myokines and understand their release mechanisms.
Main Methods:
- Quantitative proteomic analysis of circulating proteins after cycling exercise.
- Pulse-chase and intravital imaging to track EV localization.
- Arteriovenous balance studies across contracting human limbs.
Main Results:
- Over 300 proteins increased in circulation post-exercise, with enrichment in exosomal and vesicular proteins.
- Exercise-derived EVs showed localization in the liver and potential for cargo transfer.
- Novel candidate myokines were identified, released independently of classical secretion.
Conclusions:
- Exercise induces a significant release of EV-encapsulated proteins, impacting systemic signaling.
- EVs represent a key mechanism for exercise-mediated inter-tissue communication.
- This study reveals a new paradigm of exercise adaptation through novel myokine release and EV function.
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