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Related Experiment Video

Updated: Feb 15, 2026

Quantification of Circular RNAs Using Digital Droplet PCR

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

Analysis of Circular RNAs Using the Web Tool CircInteractome.

Amaresh C Panda¹, Dawood B Dudekula¹, Kotb Abdelmohsen²

¹Laboratory of Genetics and Genomics, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 12, 2018

Summary

Circular RNAs (circRNAs), stable RNA molecules, interact with microRNAs and RNA-binding proteins to regulate gene expression. The CircInteractome tool aids in exploring these interactions and designing RNA detection and silencing strategies.

Keywords:

CLIP-seq Cell- and tissue-specific circRNAs Divergent primer design Gene-specific circRNAs RNA-binding proteins Transcriptome

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Last Updated: Feb 15, 2026

Quantification of Circular RNAs Using Digital Droplet PCR

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Circular RNAs (circRNAs) are formed via back splicing, resulting in high stability compared to linear RNAs.
circRNAs play regulatory roles by interacting with microRNAs (miRNAs) and RNA-binding proteins (RBPs), influencing gene expression.

Purpose of the Study:

To introduce and review the CircInteractome web tool.
To highlight the tool's functionalities for exploring circRNA interactions and designing RNA-specific primers and siRNAs.
To provide an updated overview of the CircInteractome database.

Main Methods:

Development and utilization of the CircInteractome web tool.
Database curation for circRNA interactions with miRNAs and RBPs.
Integration of primer and siRNA design functionalities.

Main Results:

The CircInteractome tool facilitates exploration of circRNA-RBP and circRNA-miRNA interactions.
The tool enables the design of divergent primers for circRNA detection and siRNAs for circRNA silencing.
Updated functionalities for studying tissue-specific, cell-specific, and gene-specific circRNAs are available.

Conclusions:

CircInteractome is a valuable, freely accessible resource for researchers studying circRNAs.
The tool supports various aspects of circRNA research, from interaction analysis to experimental design.
Recent updates enhance the tool's utility for comprehensive circRNA investigation.