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Updated: Feb 15, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
Published on: August 4, 2016
Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR
Andreas W Heumüller1, Jes-Niels Boeckel2
1Institute for Cardiovascular Regeneration, University Frankfurt, Frankfurt, Germany.
Abstract:
Polymerase chain reaction enables the detection and characterization of circular RNA expression. The use of divergent primer pairs flanking the back-splice site, being the unique sequence element of a circular RNA, enables the detection of circular RNA expression. Here we describe the basic techniques to detect different circular transcripts of a gene or one circular RNA specifically by PCR and highlight the advantages and drawbacks of both.
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