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Development of a Larval Zebrafish Infection Model for Clostridioides difficile
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Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics
Tomasz K Prajsnar1,2,3, Gareth McVicker1,2,3, Alexander Williams1,2,3
1Krebs Institute, University of Sheffield, Sheffield, UK.
Methods in Molecular Biology (Clifton, N.J.)
|January 12, 2018
Summary
This study details protocols for examining bacterial infection dynamics in zebrafish. Researchers can now better understand pathogenesis by tracking bacterial populations within the host organism.
Area of Science:
- Microbiology
- Infectious Diseases
- Zebrafish Models
Background:
- Understanding bacterial pathogenesis requires studying bacterial dynamics within a host.
- Larval zebrafish are a valuable model organism for infection studies.
Purpose of the Study:
- To present detailed protocols for studying bacterial dynamics in embryonic zebrafish.
- To provide methods for maintaining zebrafish, performing bacterial injections, and imaging infections.
Main Methods:
- Embryonic zebrafish maintenance and handling.
- Morpholino oligonucleotide injections and intravenous bacterial suspension injections.
- Fluorescence imaging and within-host bacterial population dynamics analysis.
Main Results:
- Established protocols enable robust investigation of bacterial infection in zebrafish.
- Methods allow for detailed observation of bacterial spread and population changes within the host.
Conclusions:
- The presented protocols facilitate the study of bacterial pathogenesis using zebrafish models.
- These techniques are crucial for advancing our understanding of host-pathogen interactions.
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