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A Method to Evaluate Persistent Mycobacterium tuberculosis In Vitro and in the Cornell Mouse Model of Tuberculosis

Yanmin Hu1, Anthony Coates2

  • 1Institute of Infection and Immunity, St George's, University of London, Cranmer Terrace, London, UK. ymhu@sgul.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|January 12, 2018
PubMed

Insights

Persistent Mycobacterium tuberculosis, a dormant form of the bacteria, requires resuscitation promoting factors (RPF) to grow. This study details RPF production and its use to stimulate persistent bacteria growth in vitro and in a tuberculosis model.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Tuberculosis Research

Background:

  • Persistent Mycobacterium tuberculosis (Mtb) strains are non-replicating and resistant to standard antibiotic treatments.
  • These dormant Mtb persisters are a major hurdle for tuberculosis eradication.
  • Current culture methods fail to cultivate Mtb persisters.

Purpose of the Study:

  • To produce Resuscitation Promoting Factors (RPF) rich culture supernatants.
  • To evaluate the efficacy of RPF in stimulating the growth of Mtb persisters.
  • To assess RPF's utility in the Cornell model of tuberculosis.

Main Methods:

  • Production of RPF-rich culture supernatants from Mtb.
  • In vitro culture of Mtb persisters supplemented with RPF.
  • Application of RPF in the Cornell mouse model of tuberculosis.

Main Results:

  • RPF rich supernatants were successfully produced.
  • RPF significantly stimulated the in vitro growth of Mtb persisters.
  • RPF demonstrated efficacy in promoting Mtb persister growth within the Cornell model.

Conclusions:

  • Resuscitation Promoting Factors (RPF) are essential for Mtb persister resuscitation.
  • RPF-rich supernatants provide a viable method for cultivating Mtb persisters.
  • This approach holds potential for developing novel therapeutic strategies against tuberculosis.
Keywords:
Cornell modelCulture supernatantsMycobacterium tuberculosisPersistersResuscitation promoting factors

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