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Related Experiment Video

Updated: Feb 15, 2026

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A pH responsive AIE probe for enzyme assays.

Leilei Shi1, Yufeng Liu1, Qian Wang1

  • 1Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, Jiangsu 210093, China. weihui@nju.edu.cn.

The Analyst
|January 12, 2018
PubMed
Summary
This summary is machine-generated.

A novel pH-sensitive fluorescent probe, TPE-Leu, was developed using leucine and tetraphenylethene. This probe exhibits tunable fluorescence for detecting pH changes and enables sensitive detection of urease and acetylcholinesterase (AChE).

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Aggregation-induced emission (AIE) probes offer unique fluorescence properties.
  • Developing selective and sensitive probes for biological applications is crucial.
  • pH fluctuations play a significant role in various biological processes.

Purpose of the Study:

  • To design and synthesize a novel pH-sensitive AIE probe, TPE-Leu.
  • To investigate the pH-responsive fluorescence behavior of TPE-Leu.
  • To establish TPE-Leu as a sensing platform for enzyme detection.

Main Methods:

  • Synthesis of TPE-Leu by combining leucine and tetraphenylethene.
  • Characterization of TPE-Leu's photophysical properties under varying pH conditions.
  • Evaluation of TPE-Leu's selectivity in the presence of interfering species.
  • Application of TPE-Leu for the detection of urease and acetylcholinesterase (AChE).

Main Results:

  • TPE-Leu demonstrated significant fluorescence changes in response to pH variations, with weak fluorescence at acidic pH and strong fluorescence at basic pH.
  • The probe exhibited high selectivity for pH detection, unaffected by metal ions, redox agents, or biomolecules.
  • TPE-Leu enabled sensitive and selective detection of urease and AChE activities.
  • TPE-NH2 showed no obvious pH-sensitive properties, highlighting the role of leucine.

Conclusions:

  • TPE-Leu is a promising pH-sensitive fluorescent probe with excellent selectivity and sensitivity.
  • The developed probe offers a new strategy for designing fluorescent biosensors.
  • This work expands the application scope of AIE probes in bioassays.