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Updated: Feb 15, 2026
Model of Ischemia and Reperfusion Injury in Rabbits
Published on: November 3, 2023
Effects of molsidomine on retinal ischemia/reperfusion injury in rabbits
N Polat1, M A Ozer2, H Parlakpinar3
1a Department of Ophthalmology , Inonu University School of Medicine , Malatya.
Abstract:
We investigated the effect of molsidomine (MOL) on ischemia/reperfusion (I/R) injury. Rabbits were assigned to four groups: group 1, sham; group 2, I/R; group 3, MOL treatment for 4 days after I/R; group 4, MOL treatment for 1 day before I/R and 3 days after I/R. Retinal I/R was produced by elevating the intraocular pressure to 150 mm Hg for 60 min. Seven days after I/R, the eyes were enucleated. Retinal changes were examined using histochemistry. The levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) also were measured. We found a significant increase in the thickness of the outer nuclear layer of group 3 compared to the other groups. In groups 3 and 4, caspase-3 stained cells in the ganglion cell layer were decreased compared to group 2. We found a significant increase in caspase-3 stained cells in the inner nuclear layer (INL) of group 2 compared to the other groups. We found a significant increase in caspase-3 stained cells in group 3 compared to group 4 in the INL. The MDA level in group 2 was significantly higher than group 1 and MOL significantly decreased MDA levels in groups 3 and 4. We found that MOL protected the retina from I/R injury by enhancing antioxidative effects and inhibiting apoptosis of retinal cells.
Insights
Molsidomine (MOL) protects the retina from ischemia/reperfusion (I/R) injury by reducing oxidative stress and inhibiting retinal cell apoptosis. This study demonstrates MOL
Area of Science:
- Ophthalmology
- Neuroscience
- Pharmacology
Background:
- Ischemia/reperfusion (I/R) injury is a significant cause of retinal damage.
- Oxidative stress and apoptosis play crucial roles in the pathogenesis of retinal I/R injury.
- Molsidomine (MOL) is a potential therapeutic agent for I/R-related conditions.
Purpose of the Study:
- To investigate the protective effects of molsidomine (MOL) against retinal ischemia/reperfusion (I/R) injury in a rabbit model.
- To evaluate the impact of MOL on oxidative stress markers and apoptotic pathways in the retina following I/R.
- To determine the optimal timing and duration of MOL treatment for mitigating retinal I/R damage.
Main Methods:
- Retinal I/R injury was induced in rabbits by elevating intraocular pressure.
- Rabbits were divided into four groups: sham, I/R, and two MOL treatment groups (post-I/R and pre/post-I/R).
- Retinal tissues were analyzed using histochemistry to assess cellular changes and apoptosis (caspase-3 staining), and biochemical assays measured oxidative stress markers (MDA, GSH, SOD, GSH-Px, CAT).
Main Results:
- MOL treatment significantly reduced caspase-3 positive cells in the ganglion cell layer and inner nuclear layer (INL) compared to the I/R group.
- MOL administration significantly decreased malondialdehyde (MDA) levels, an indicator of lipid peroxidation, in the retina.
- Histochemical analysis revealed reduced retinal damage and thickness in the outer nuclear layer in MOL-treated groups.
- MOL treatment enhanced antioxidant enzyme activity, indicated by restored levels of GSH, SOD, GSH-Px, and CAT.
Conclusions:
- Molsidomine (MOL) demonstrates significant protective effects against retinal ischemia/reperfusion (I/R) injury.
- MOL exerts its protective effects by enhancing the retina's antioxidative capacity and inhibiting apoptosis of retinal cells.
- These findings suggest MOL as a promising therapeutic candidate for managing retinal I/R injury.
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