Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

pre-mRNA Processing02:01

pre-mRNA Processing

57.7K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.7K
Concentration Cells02:41

Concentration Cells

25.9K
A concentration cell is a type of a  voltaic cell constructed by connecting two almost identical half-cells, both based on the same half-reaction and using the same electrode, differing only in the concentration of one redox species. A concentration cell's potential, therefore, is determined only by the concentration difference of the particular redox species.
Consider the following voltaic cell:
25.9K
Development of Analytical Methods01:21

Development of Analytical Methods

2.4K
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
2.4K
Performing a Simple Data Analysis using MS-Excel Function01:17

Performing a Simple Data Analysis using MS-Excel Function

1.1K
Microsoft Excel offers a suite of functions and tools ideal for statistical analysis, making it accessible to students and researchers. This article outlines fundamental Excel functions pivotal for data analysis.
SUM: This function calculates the total sum of a range of values. It's the foundation for aggregating data, essential for determining overall trends and totals in datasets.
AVERAGE: It computes the mean value of a given set of numbers, providing a quick insight into the central...
1.1K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

15.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
15.8K
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

2.8K
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional connectivity changes in patients with absence epilepsy studied using resting-state functional MRI.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2012
Same author

Quantitative analysis of mitral valve morphology in mitral valve prolapse with real-time 3-dimensional echocardiography: importance of annular saddle shape in the pathogenesis of mitral regurgitation.

Circulation·2012
Same author

Interval cancers in nasopharyngeal carcinoma screening: comparing two screening intervals after a negative initial screening result.

Journal of medical screening·2012
Same author

Two Epstein-Barr virus-related serologic antibody tests in nasopharyngeal carcinoma screening: results from the initial phase of a cluster randomized controlled trial in Southern China.

American journal of epidemiology·2012
Same author

MALT1 small molecule inhibitors specifically suppress ABC-DLBCL in vitro and in vivo.

Cancer cell·2012
Same author

Extraction and characterization of extracellular polymeric substances in biofilm and sludge via completely autotrophic nitrogen removal over nitrite system.

Applied biochemistry and biotechnology·2012

Related Experiment Video

Updated: Feb 15, 2026

Fabricating Cotton Analytical Devices
05:40

Fabricating Cotton Analytical Devices

Published on: August 30, 2016

7.1K

Simultaneous pre-concentration and separation on simple paper-based analytical device for protein analysis.

Ji-Cheng Niu1, Ting Zhou1, Li-Li Niu2

  • 1Research Center for Analytical Sciences, Chemistry Department College of Sciences, Northeastern University, Shenyang, Liaoning, 110819, China.

Analytical and Bioanalytical Chemistry
|January 13, 2018
PubMed
Summary

This study introduces fast isoelectric focusing (IEF) on paper fluidics for rapid protein separation and concentration. This cost-effective method enables quick biomarker analysis and point-of-care testing applications.

Keywords:
Isoelectric focusing (IEF)Paper-based analytical device (PAD)Point-of-care test (POCT)Protein sample pretreatment

More Related Videos

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

12.0K
Thermal Measurement Techniques in Analytical Microfluidic Devices
08:29

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

10.1K

Related Experiment Videos

Last Updated: Feb 15, 2026

Fabricating Cotton Analytical Devices
05:40

Fabricating Cotton Analytical Devices

Published on: August 30, 2016

7.1K
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

12.0K
Thermal Measurement Techniques in Analytical Microfluidic Devices
08:29

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

10.1K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Microfluidics

Background:

  • Isoelectric focusing (IEF) is a powerful protein separation technique.
  • Traditional IEF can be time-consuming and requires specialized equipment.
  • Need for rapid and accessible protein analysis methods, especially for point-of-care applications.

Purpose of the Study:

  • To develop a fast and efficient isoelectric focusing (IEF) method using open paper fluidics.
  • To demonstrate simultaneous protein concentration and separation from complex biological matrices.
  • To evaluate the potential of this method for point-of-care testing (POCT) and biomarker analysis.

Main Methods:

  • Implementation of fast isoelectric focusing (IEF) on an open paper fluidic channel.
  • Utilized smartphone-based colorimetric detection for estimating protein concentration and resolution.
  • Performed off-line identification of proteins using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

Main Results:

  • Achieved IEF separation in just 10 minutes.
  • Obtained a high resolution of 0.03 pH units.
  • Demonstrated a concentration factor of 10 for model proteins.
  • Successfully detected albumin in human serum and glycated hemoglobin (HbA1c) in blood cells.
  • Confirmed protein identification from IEF fractions via MALDI-TOF-MS.

Conclusions:

  • Fast IEF on paper fluidics offers a rapid and effective method for protein concentration and separation.
  • This technique provides a cost-effective sample pretreatment solution for biomarker analysis.
  • The developed paper-based IEF (PAD IEF) shows significant potential for point-of-care diagnostic applications.