Related Experiment Video
Updated: Feb 15, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Published on: October 9, 2014
Relative sensitivity variation law in the field of fluorescence intensity ratio thermometry
Abstract:
We study the variation law of relative sensitivity in the field of fluorescence intensity ratio thermometry. It is theoretically demonstrated that there must be only one maximum value of relative sensitivity in the case in which there is a positive offset in fitting function. Moreover, the method to obtain this maximum is proposed. Experimental results, taking the D15/D50 levels of Eu3+ as examples, are in excellent accordance with the conclusion. The mechanism behind is then investigated, and other populating processes imposed on the D15 level, which exert negative outcome on thermal sensitivity, are found to play a key role in determination of this unique variation law.
Related Concept Videos
Relation between Poisson's ratio, Modulus of Elasticity and Modulus of Rigidity
What is Variation?
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
Gas Laws: Boyle's, Gay-Lussac, Charles', Avogadro's, and Ideal Gas Law
Hess's Law
Third Law of Thermodynamics
Second Law of Thermodynamics

