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Updated: Feb 15, 2026
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Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
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Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects
Hana Raschmanová1, Astrid Weninger2, Anton Glieder2
1Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 16628 Prague, Czech Republic.
Biotechnology Advances
|January 15, 2018
Summary
The CRISPR-Cas system revolutionizes genome and metabolic engineering in various yeast species. This study compares CRISPR-Cas expression strategies to enhance genome editing efficiency in eukaryotes.
Area of Science:
- Molecular Biology
- Synthetic Biology
- Biotechnology
Background:
- The CRISPR-Cas system has rapidly become a primary tool for genome engineering.
- It has significantly accelerated and improved metabolic engineering in both conventional and non-conventional yeasts.
- Advanced CRISPR applications like crisprTFs and gene drives are well-established, particularly in Saccharomyces cerevisiae.
Purpose of the Study:
- To compare innovative CRISPR-Cas expression strategies in yeasts.
- To provide a guideline for implementing and refining CRISPR-Cas systems for efficient genome editing.
- To highlight the versatility of CRISPR-Cas in diverse eukaryotic organisms.
Main Methods:
- Comparative analysis of CRISPR-Cas expression strategies.
- Evaluation of CRISPR-Cas system efficiency in various yeast species (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Yarrowia lipolytica, Pichia pastoris, Kluyveromyces lactis, Candida albicans, Candida glabrata).
Main Results:
- CRISPR-Cas systems demonstrate significant impact on genome and metabolic engineering speed and efficiency across multiple yeast types.
- Established advanced applications in S. cerevisiae include crisprTFs and gene drives.
- The comparison offers insights into optimizing CRISPR-Cas for robust genome editing.
Conclusions:
- CRISPR-Cas is a transformative technology for yeast engineering.
- Optimized expression strategies are crucial for maximizing CRISPR-Cas efficiency.
- This work serves as a foundational guide for applying CRISPR-Cas in yeast and other eukaryotes.
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