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Updated: Feb 15, 2026

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
Published on: October 11, 2012
Analysis of Mitochondrial Markers of Programmed Cell Death
Dong Xiao1, Huyi He1, Wenjing Huang1
1College of Agronomy, Guangxi University, Nanning, Guangxi, People's Republic of China.
Abstract:
Mitochondria play a crucial role in programmed cell death (PCD) in plants. In most cases of mitochondria-dependent PCD, cytochrome c (Cyt c) released from mitochondria due to the opening of mitochondrial permeability transition pore (MPTP) and the activation of caspase-like proteases. Here we describe the analytic methods of mitochondrial markers of PCD including mitochondria isolation, mitochondrial membrane permeability, mitochondrial inner membrane potential, Cytc release, ATP, and mitochondrial reactive oxygen species (ROS).
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