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Human parvovirus B19 and parvovirus 4 among Iranian patients with hemophilia
Davod Javanmard1, Masood Ziaee2, Hadi Ghaffari1
1Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Background:
Human parvovirus B19 (B19V) is one of the smallest DNA viruses and shows great resistance to most disinfectants. Therefore, it is one of the common contaminant pathogens present in blood and plasma products. Parvovirus 4 (PARV4) is a newly identified parvovirus, which is also prevalent in parenteral transmission. In this study, we aimed to evaluate the prevalence of B19V and PARV4 DNA among patients with hemophilia in Birjand County in eastern Iran.
Methods:
This was a cross-sectional epidemiological study comprising nearly all people with hemophilia in this region. Whole blood samples were taken after patient registration and sent for plasma isolation. After nucleic acid extraction, B19V was detected with real-time polymerase chain reaction, PARV4 DNA was then detected using sensitive semi-nested PCR.
Results:
In total, there were 86 patients with hemophilia, with mean age 28.5±1.5 years. Of these, 90.7% were men and 9.3% women; 84.9% had hemophilia A and 7.0% had hemophilia B. We found 11 patients (12.8%) were positive for B19V DNA and 8 were positive (9.3%) for PARV4 DNA. The prevalence of B19V was higher in middle-aged groups rather than younger people, whereas PARV4 infection was more common in younger patients (P <0.05).
Conclusion:
There was a high prevalence of B19V and PARV4 infection in this high-risk group of patients with hemophilia. Due to the clinical significance of the B19 virus, imposing more precautionary measures for serum and blood products is recommended.
Insights
Prevalence of Human parvovirus B19 (B19V) and Parvovirus 4 (PARV4) DNA was high in Iranian patients with hemophilia. B19V was more common in middle-aged individuals, while PARV4 was more prevalent in younger patients.
Area of Science:
- Virology
- Epidemiology
- Hematology
Background:
- Human parvovirus B19 (B19V) is a disinfectant-resistant DNA virus, frequently contaminating blood products.
- Parvovirus 4 (PARV4) is a newly identified parvovirus also transmitted parenterally.
Purpose of the Study:
- To determine the prevalence of B19V and PARV4 DNA in hemophilia patients in Birjand County, Iran.
- To investigate potential age-related differences in the prevalence of these viral infections within this cohort.
Main Methods:
- A cross-sectional epidemiological study involving hemophilia patients in Birjand County.
- Real-time PCR used for B19V detection and semi-nested PCR for PARV4 DNA detection in whole blood samples.
Main Results:
- Out of 86 hemophilia patients, 12.8% tested positive for B19V DNA and 9.3% for PARV4 DNA.
- B19V prevalence increased with age, whereas PARV4 was more common in younger patients (P <0.05).
Conclusions:
- A significant prevalence of B19V and PARV4 infections was observed in this high-risk hemophilia population.
- Enhanced precautionary measures for blood and serum products are recommended due to B19V's clinical importance.
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