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Updated: Feb 15, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
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Lactic Acid Bacteria May Impact Intestinal Barrier Function by Modulating Goblet Cells.

Chengcheng Ren1,2, Jelleke Dokter-Fokkens1, Susana Figueroa Lozano1

  • 1Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Hanzeplein 1, The Netherlands.

Molecular Nutrition & Food Research
|January 16, 2018
PubMed
Summary

Lactic acid bacteria (LAB) strains and species differentially impact intestinal goblet cell mucus production. Specific LAB can modulate mucus synthesis genes, offering targeted gut health benefits.

Keywords:
bioactive food componentsgoblet cellsintestinal mucus barrierlactic acid bacteriamodulatory property

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Area of Science:

  • Microbiology
  • Gastroenterology
  • Cell Biology

Background:

  • Lactic acid bacteria (LAB) are known to promote gastrointestinal health.
  • Mechanisms underlying LAB's effects on the gut barrier, particularly mucus modulation, require further elucidation.
  • Goblet cells are crucial for intestinal mucus production and barrier function.

Purpose of the Study:

  • To investigate the impact of different LAB strains and species on genes involved in goblet cell mucus synthesis.
  • To determine if LAB-derived factors can directly influence the expression of mucus-associated genes.
  • To assess LAB effects under conditions mimicking gut inflammation or stress.

Main Methods:

  • Studied gene expression of mucin MUC2, trefoil factor 3, resistin-like molecule β, carbohydrate sulfotransferase 5, and galactose-3-O-sulfotransferase 2 in the human goblet cell line LS174T.
  • Exposed cells to various LAB strains or their conditioned media.
  • Assessed gene transcription with and without inflammatory cytokines (TNF-α, IL-13) or tunicamycin.

Main Results:

  • LAB demonstrated species- and strain-specific effects on goblet cell mucus-related gene expression.
  • LAB's influence on these genes varied in the presence of cytokines or tunicamycin.
  • Secreted bioactive factors from certain LAB strains were found to regulate goblet cell gene expression.

Conclusions:

  • Findings reveal distinct modulatory effects of different LAB species and strains on mucus synthesis genes.
  • This study elucidates direct interactions between LAB and intestinal goblet cells.
  • Highlights the importance of selecting specific LAB for targeted gut health benefits.