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Updated: Feb 15, 2026

Measuring Associative Learning in Chemotaxis of the Nematode Caenorhabditis elegans
Published on: June 17, 2025
Behavioral Assays to Study Oxygen and Carbon Dioxide Sensing in Caenorhabditis elegans
Teresa Rojo Romanos1,2, Leelee Ng1, Roger Pocock1,2
1Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia.
Abstract:
Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, we describe a C. elegans population behavioral assay used to measure locomotory responses to changes in environmental oxygen (O2) and carbon dioxide (CO2) concentrations. These behavioral assays are high-throughput and enable examination of genetic, neuronal and circuit function.
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