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Related Concept Videos

Data Collection II01:29

Data Collection II

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The nursing history captures and records the patient's health status, so that a care plan evolves to meet the patient's individual needs. The nursing health history is a part of the initial assessment. A comprehensive history covers all health dimensions and plays a significant role in the assessment process. A comprehensive history includes the patient's biographical information, reasons for seeking health care, expectations, present and past health history, medications, and...
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Data Collection I01:30

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Data collection gathers information needed to make accurate judgments about a patient's present condition. During a health history interview, subjective data is collected from the patient, their caregivers, or family members, and objective data is collected through observations and physical assessment. Patients are the primary source of subjective data. Thus information gathered from patients through interviews, observations, and physical examination is primary data. Secondary sources of...
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Data Collection by Experiments01:13

Data Collection by Experiments

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Data collection is a systematic method of obtaining, observing, measuring, and analyzing accurate information. An experimental study is a standard method of data collection that involves the manipulation of the samples by applying some form of treatment prior to data collection. It refers to manipulating one variable to determine its changes on another variable. The sample subjected to treatment is known as “experimental units.”
An example of the experimental method is a public...
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Data Collection by Survey01:07

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The systematic method of obtaining and analyzing accurate information of a population is called data collection. A survey is a standard method of data collection that involves collecting information from a target human population about their experience, opinion, or knowledge of a product, service, or process. The responses are recorded and interpreted. The most common survey examples are written questionnaires, face-to-face or telephonic conversations, focus groups, and electronic (e-mail or...
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Data Collection by Observations01:08

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Data collection refers to a systematic way of obtaining, observing, measuring, and analyzing accurate information. Observational studies are one of the most widely used methods of data collection. It involves collecting data by observing the behavior and physical characteristics of a sample without making any modifications to the sample.
An astronomer viewing the motion and brightness of stars in the sky and recording the data is an example of observational data collection. A botanist recording...
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Data Collection III01:05

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The physical assessment examines the patient for objective data that defines the patient's condition, and aids in formulating the nursing care plan. The purpose of physical assessment is a health status appraisal, which includes identifying health problems, and establishing a database for nursing intervention.
The principles to begin the physical assessment include conducting a comprehensive or problem-related history in a quiet, well-lit room, emphasizing privacy and comfort for the...
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Related Experiment Video

Updated: Feb 15, 2026

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
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Volta phase plate data collection facilitates image processing and cryo-EM structure determination.

Ottilie von Loeffelholz1, Gabor Papai1, Radostin Danev2

  • 1Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), 1 rue Laurent Fries, Illkirch, France; Centre National de la Recherche Scientifique (CNRS) UMR 7104, Illkirch, France; Institut National de la Santé et de la Recherche Médicale (Inserm) U1258, Illkirch, France; Université de Strasbourg, Strasbourg, France.

Journal of Structural Biology
|January 17, 2018
PubMed
Summary
This summary is machine-generated.

Volta phase plates (VPP) significantly enhance contrast in cryo-electron microscopy (cryo-EM) imaging. This study demonstrates VPP improves data processing and structure refinement, requiring fewer images for high-resolution reconstructions.

Keywords:
Cryo electron microscopyHigh-resolution cryo-EMImage processingRibosomeStructural biologyVolta phase plate

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Area of Science:

  • Structural Biology
  • Biophysics
  • Microscopy

Background:

  • Cryo-electron microscopy (cryo-EM) faces challenges in high-resolution structure determination of macromolecular complexes.
  • Obtaining sufficient data for accurate 3D reconstructions and visualizing small, sub-stoichiometric ligands remains a bottleneck.
  • Volta phase plates (VPP) enhance image contrast in low-dose cryo-EM by introducing a phase shift, preserving high-frequency information.

Purpose of the Study:

  • To comparatively analyze the behavior of different datasets during image processing in cryo-EM.
  • To quantify key parameters during structure refinement using Volta phase plates.
  • To evaluate the impact of VPP on cryo-EM data processing and structural biology.

Main Methods:

  • Automated data collection from a human ribosome sample using conventional defocus, close-to-focus VPP (cfVPP), and small-defocus VPP (dfVPP).
  • Comparative analysis of image processing parameters, including particle alignment, Euler angle assignment, and 2D/3D classification.
  • Quantification of resolution achieved with fewer particle images using VPP datasets.

Main Results:

  • Data collected with small-defocus VPP (dfVPP) demonstrated more robust performance during cryo-EM structure refinement.
  • dfVPP datasets showed increased stability and faster convergence in particle alignment, angle assignment, and classification.
  • Fewer particle images were required to achieve comparable resolution in 3D reconstructions with VPP compared to conventional methods.
  • Conventional defocus range data collection is also compatible with VPP technology.

Conclusions:

  • Volta phase plate cryo-EM significantly facilitates data processing and interpretation, including atomic model refinement.
  • VPP technology offers a substantial improvement for structural biology, enabling more efficient high-resolution structure determination.
  • The findings highlight the potential of VPP to overcome current bottlenecks in cryo-EM data acquisition and analysis.