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Avian Retrovirus-Mediated Tumor-Specific Gene Knockout

Wei Wang1, Bingning Dong1, Feng Yang1

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas.

Current Protocols in Molecular Biology
|January 17, 2018
PubMed

Insights

We developed a novel gene delivery system, RCAS-Cre-IRES-Oncogene (RCI-Oncogene), for in vivo tumor gene knockout. This system demonstrated FGFR1

Area of Science:

  • Oncology
  • Gene Therapy
  • Cancer Research

Background:

  • The replication-competent avian sarcoma leukosis virus long-terminal repeat with splice acceptor-tumor virus A (RCAS-TVA) system is established for cancer modeling.
  • Spontaneous tumor gene manipulation in vivo requires efficient and versatile gene delivery methods.

Purpose of the Study:

  • To introduce a novel gene delivery system, RCAS-Cre-IRES-Oncogene (RCI-Oncogene), for precise gene manipulation in spontaneous tumors.
  • To establish a tumor gene knockout (TuKO) protocol using the RCI-Oncogene system.
  • To investigate the role of FGFR1 in mammary tumor metastasis.

Main Methods:

  • Development of the RCI-Oncogene system for efficient in vivo gene delivery.
  • Application of the RCI-Oncogene system for tumor gene knockout (TuKO).
  • Utilizing mutant mouse hosts for targeted gene modification in tumors.

Main Results:

  • The RCI-Oncogene system enables efficient manipulation of gene expression in spontaneous tumors.
  • The TuKO approach using RCI-Oncogene demonstrated a critical role for FGFR1 in mammary tumor metastasis.

Conclusions:

  • The RCI-Oncogene system is a versatile tool for tumor gene modification in vivo.
  • The TuKO approach provides a robust method for studying gene function in spontaneous tumors.
  • This technology can be adapted for various research questions in appropriate mouse models.
Keywords:
CreRCASRCIRCI-OncogeneRCI-PyMTTVAtumor specific knockout

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