To Explore the Mechanism of the GRM4 Gene in Osteosarcoma by RNA Sequencing and Bioinformatics Approach

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Updated: Feb 15, 2026

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

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Yunguo Pang1,2, Jinmin Zhao2,3, Mitra Fowdur1,2

  • 1Division of Spinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China (mainland).

Medical Science Monitor Basic Research
|January 18, 2018
PubMed

Insights

Glutamate metabotropic receptor 4 (GRM4) plays a role in osteosarcoma. Silencing GRM4 identified key genes and pathways involved in osteosarcoma development, suggesting EGR1 and CTCF as regulatory factors.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Glutamate metabotropic receptor 4 (GRM4) is implicated in osteosarcoma pathogenesis.
  • Understanding GRM4's molecular mechanisms is crucial for osteosarcoma research.

Purpose of the Study:

  • To investigate the molecular mechanisms of GRM4 in osteosarcoma.
  • To identify genes and pathways regulated by GRM4 in osteosarcoma cells.

Main Methods:

  • Real-time quantitative PCR (RT-qPCR) to assess GRM4 expression.
  • RNA sequencing and bioinformatics analysis for differentially expressed genes (DEGs) after GRM4 silencing.
  • Prediction of transcription factors (TFs) and construction of protein-protein interaction (PPI) networks.

Main Results:

  • 51 significant DEGs were identified, with 14 upregulated and 37 downregulated.
  • KEGG pathway analysis revealed four significant enrichment pathways.
  • Six TFs potentially regulating GRM4 were detected; EGR1 and CTCF were highlighted.
  • A PPI network identified 182 genes enriched in 14 pathways, with chemokines and receptors in three pathways.

Conclusions:

  • DEGs in identified pathways likely contribute to osteosarcoma development via GRM4.
  • EGR1 and CTCF are potential regulators of GRM4 in osteosarcoma progression.
  • GRM4's role in osteosarcoma involves interactions with chemokines and their receptors.

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