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Updated: Feb 15, 2026

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
Published on: April 20, 2015
A well-defined unimolecular channel facilitates chloride transport
Sujun Chen1, Yongliang Zhao, Chunyan Bao
1Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130# Meilong Road, Shanghai, 200237, P. R. China. baochunyan@ecust.edu.cn linyongzhu@ecust.edu.cn.
Abstract:
A unimolecular ion channel was optimized by functionalization with a new type of rigid-rod oligomer. The macrocycle pendant endows chloride selectivity and the fluorescence feature and suitable length of the rod facilitates the visual insertion of channels into the lipid bilayer, resulting in efficient ion transport with an EC50 value of 0.36 μM.
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