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Updated: Feb 15, 2026
10:04
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
Published on: September 18, 2017
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Cornea-Derived Mesenchymal Stromal Cells Therapeutically Modulate Macrophage Immunophenotype and Angiogenic Function
Medi Eslani1, Ilham Putra1, Xiang Shen1
1Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, USA.
Stem Cells (Dayton, Ohio)
|January 18, 2018
Summary
Cornea-derived mesenchymal stromal cells (cMSCs) reduce inflammatory corneal neovascularization by altering macrophage function. These cells suppress macrophage pro-angiogenic activity, mediated by pigment epithelial derived factor (PEDF).
Area of Science:
- Ophthalmology
- Immunology
- Regenerative Medicine
Background:
- Macrophages drive inflammatory corneal neovascularization, making them therapeutic targets.
- Corneal neovascularization contributes to vision impairment.
Purpose of the Study:
- To investigate if cornea-derived mesenchymal stromal cells (cMSCs) can modulate macrophage function.
- To explore the therapeutic potential of cMSCs in treating corneal neovascularization.
Main Methods:
- In vitro and in vivo studies using cMSCs and macrophages.
- Analysis of macrophage phenotype, apoptosis, and angiogenic function.
- Assessment of corneal neovascularization in murine models.
- Investigation of pigment epithelial derived factor (PEDF) role.
Main Results:
- cMSCs induced macrophage apoptosis and promoted an anti-angiogenic immunophenotype (CD14hi CD16hi CD163hi CD206hi).
- In vivo, cMSCs reduced macrophage infiltration and increased CD206 expression in injured corneas.
- cMSC-educated macrophages exhibited reduced neovascularization in vivo.
- Pigment epithelial derived factor (PEDF) secreted by cMSCs was crucial for modulating macrophage phenotype and function.
Conclusions:
- cMSCs therapeutically suppress the angiogenic capacity of macrophages.
- cMSC-derived PEDF plays a key role in modulating macrophage phenotype and function.
- cMSCs represent a promising cell-based therapy for inflammatory corneal neovascularization.
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