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Updated: Feb 15, 2026

Grafting Multiwalled Carbon Nanotubes with Polystyrene to Enable Self-Assembly and Anisotropic Patchiness
Published on: April 1, 2018
Immobilization of cross-linked tannase enzyme on multiwalled carbon nanotubes and its catalytic behavior
Chong-Boon Ong1, Mohamad S M Annuar1
1a Faculty of Science , Institute of Biological Sciences, University of Malaya , Kuala Lumpur , Malaysia.
Abstract:
Immobilization of cross-linked tannase on pristine multiwalled carbon nanotubes (MWCNT) was successfully performed. Cross-linking of tannase molecules was made through glutaraldehyde. The immobilized tannase exhibited significantly improved pH, thermal, and recycling stability. The optimal pH for both free and immobilized tannase was observed at pH 5.0 with optimal operating temperature at 30°C. Moreover, immobilized enzyme retained greater biocatalytic activities upon 10 repeated uses compared to free enzyme in solution. Immobilization of tannase was accomplished by strong hydrophobic interaction most likely between hydrophobic amino acid moieties of the glutaraldehyde-cross-linked tannase to the MWCNT.
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