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Author Spotlight: MAPP Protocol – Advancing Glycan Analysis
Published on: September 29, 2023
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Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
Sharad Purohit1,2,3, Tiehai Li4, Wanyi Guan4
1Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, GA, 30912, USA.
Nature Communications
|January 19, 2018
Summary
A new multiplex glycan bead array (MGBA) enables high-throughput analysis of 500 glycans across 384 samples. This glycan-binding protein (GBP) research tool aids biomarker discovery, including for ovarian cancer.
Area of Science:
- Biochemistry
- Immunology
- Glycomics
Background:
- Glycan-binding proteins (GBPs) are crucial for cellular functions, including immune response and cell adhesion.
- Analyzing GBP-glycan interactions has been limited by the lack of high-throughput, high-content technologies.
Purpose of the Study:
- To develop and validate a novel multiplex glycan bead array (MGBA) platform for high-throughput glycan analysis.
- To demonstrate the utility of MGBA in large-scale human serum studies and biomarker discovery.
Main Methods:
- Development of the multiplex glycan bead array (MGBA) for simultaneous analysis of up to 500 glycans in 384 samples.
- Validation of MGBA specificity, sensitivity, and reproducibility using lectins, antibodies, and mammalian GBPs.
- Application of MGBA to analyze anti-glycan IgM and IgG antibodies in 961 human serum samples.
Main Results:
- MGBA successfully analyzed 39 plant lectins, 13 recombinant anti-glycan antibodies, and mammalian GBPs.
- The platform enabled the analysis of natural anti-glycan antibodies in a cohort of 961 human serum samples.
- MGBA facilitated the discovery of anti-glycan antibody biomarkers associated with ovarian cancer.
Conclusions:
- The multiplex glycan bead array (MGBA) is a robust platform for high-throughput glycan analysis.
- MGBA is well-suited for large population-based studies and biomarker discovery in glycomics and immunology.
- This technology advances the study of glycan-binding protein interactions and their roles in disease.
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