Related Experiment Video
Updated: Feb 15, 2026
09:56
Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
Published on: August 26, 2025
656
Application of Genome Editing Techniques in Immunology
Agata O Zych1,2, Malgorzata Bajor1, Radoslaw Zagozdzon3,4,5
1Department of Immunology, Medical University of Warsaw, Warsaw, Poland.
Archivum Immunologiae Et Therapiae Experimentalis
|January 19, 2018
Summary
Adoptive cell therapies engineer immune cells to fight cancer. Novel genetic engineering tools like TALEN and CRISPR/Cas9 enhance these therapies by enabling precise gene editing for improved cancer treatment and broader disease applications.
Area of Science:
- Immunology
- Biotechnology
- Genetics
Background:
- Adoptive cell therapy is a promising cancer treatment using genetically engineered immune cells.
- Engineered T cells with cancer-targeted receptors show clinical responses.
- Gene disruption is crucial for enhancing cell therapies and studying pathways.
Purpose of the Study:
- To review novel genetic engineering methods for immune cell modification.
- To highlight the role of TALEN and CRISPR/Cas9 in advancing cell therapies.
- To explore potential applications beyond cancer treatment.
Main Methods:
- Review of genetic engineering techniques, focusing on TALEN and CRISPR/Cas9.
- Discussion of gene disruption strategies in immune cells.
- Analysis of applications in adoptive cell therapy.
Main Results:
- TALEN and CRISPR/Cas9 offer precise gene editing capabilities for immune cells.
- These methods facilitate the development of more potent and targeted cell therapies.
- Potential for application in treating various diseases.
Conclusions:
- Novel genetic engineering tools significantly advance adoptive cell therapy.
- CRISPR/Cas9 and TALEN enable enhanced immune cell functions for cancer treatment.
- These technologies hold promise for future therapeutic strategies in oncology and beyond.
Related Concept Videos
01:28CRISPR/Cas9 Genome Editing
2.0K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.0K
02:23RNA Editing
9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
02:02Genomics
40.9K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
01:23Immunological Memory
17.1K
Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
17.1K
02:30Genomic Imprinting and Inheritance
37.3K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.3K
03:21Genome Size and the Evolution of New Genes
9.2K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.2K