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Live-Animal Imaging of Renal Function by Multiphoton Microscopy
Kenneth W Dunn1, Timothy A Sutton1, Ruben M Sandoval1
1Indiana University School of Medicine, Indianapolis, Indiana.
Current Protocols in Cytometry
|January 19, 2018
Summary
Intravital microscopy allows high-resolution imaging of living kidneys. This technique uses multiphoton fluorescence microscopy and probes to study kidney structure and function in real-time.
Area of Science:
- Physiology
- Microscopy
- Renal Biology
Background:
- Intravital microscopy bridges cell culture resolution with in vivo relevance.
- Recent advances include multiphoton fluorescence microscopy for deep tissue imaging.
Purpose of the Study:
- To detail the application of multiphoton intravital microscopy for studying the living kidney.
- To highlight its capability for subcellular resolution imaging of renal structures.
Main Methods:
- Administering fluorescent probes to anesthetized, surgically prepared animals.
- Acquiring images of the kidney using multiphoton fluorescence microscopy for up to 3 hours.
- Utilizing specialized computer systems for digital image analysis of acquired data.
Main Results:
- High-resolution characterization of glomeruli, tubules, and vasculature in the living kidney.
- Evaluation of dynamic processes like glomerular and vascular permeability.
- Assessment of cellular functions including endocytosis and mitochondrial activity.
Conclusions:
- Multiphoton intravital microscopy is a powerful tool for in vivo renal research.
- This technique enables detailed study of kidney structure and function at subcellular resolution.
- It supports diverse applications for evaluating kidney physiology and pathology.
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