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Related Experiment Video

Updated: Feb 15, 2026

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Enhancer-associated long non-coding RNA LEENE regulates endothelial nitric oxide synthase and endothelial function.

Yifei Miao1, Nassim E Ajami2, Tse-Shun Huang3

  • 1Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope, 1500 Duarte Rd., Duarte, CA, 91010, USA.

Nature Communications
|January 20, 2018
PubMed
Summary
This summary is machine-generated.

Researchers discovered a novel long non-coding RNA, LEENE, that enhances endothelial nitric oxide synthase (eNOS) expression. This finding reveals a new epigenetic mechanism crucial for endothelial function and cardiovascular health.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cardiovascular Research

Background:

  • Endothelial nitric oxide synthase (eNOS) is critical for vascular homeostasis and function.
  • eNOS expression is regulated at multiple levels, but epigenetic mechanisms involving long non-coding RNAs (lncRNAs) are underexplored.

Purpose of the Study:

  • To identify and characterize novel epigenetic regulators of eNOS expression.
  • To elucidate the role of lncRNAs in modulating eNOS and endothelial cell function.

Main Methods:

  • RNA-sequencing and chromatin conformation capture (3C) were employed to identify and analyze lncRNAs.
  • Gain- and loss-of-function experiments were performed to assess the impact of LEENE on eNOS expression and endothelial cell function.

Main Results:

  • An enhancer-associated lncRNA, named LEENE, was identified and shown to be co-regulated with eNOS.
  • LEENE enhances eNOS expression by facilitating RNA Polymerase II recruitment to the eNOS promoter, boosting transcription.
  • Modulation of LEENE levels directly impacts eNOS expression and endothelial cell function.

Conclusions:

  • LEENE represents a novel epigenetic regulator of eNOS, adding a new layer to our understanding of endothelial homeostasis.
  • These findings offer new insights into cardiovascular regulation and potential therapeutic targets involving endothelial function.