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Updated: Feb 15, 2026

Multiscale Structures Aggregated by Imprinted Nanofibers for Functional Surfaces
Published on: September 11, 2018
Three-dimensional multiscale fiber matrices: development and characterization for increased HepG2 functional
Surendra Kumar Verma1, Akshay Modi, Jayesh Bellare
1Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai - 400076, India. jb@iitb.ac.in.
Researchers developed advanced 3D fiber-based scaffolds using polycaprolactone, chitosan, and gelatin. These biocompatible scaffolds enhance cell adhesion and function, showing promise for tissue engineering and artificial liver devices.
Area of Science:
- Tissue Engineering
- Biomaterials Science
- Regenerative Medicine
Background:
- Developing nature-like cell-growth substrates is crucial for tissue engineering.
- Existing substrates often struggle to fully support cell adhesion and functional maintenance.
- A microenvironment mimicking native tissues is needed for effective cell proliferation and function.
Purpose of the Study:
- To develop novel three-dimensional (3D) micro-nano multiscale fiber-based substrates.
- To evaluate the physico-chemical properties, hemocompatibility, and cytotoxicity of these substrates.
- To assess the cellular functionality of HepG2 cells cultured on the developed matrices for potential bioreactor applications.
Main Methods:
- Fabrication of 3D micro-nano multiscale fiber-based substrates by electrospinning polycaprolactone (PCL), PCL-Chitosan (C), and PCL-Chitosan-Gelatin (G) nanofibers onto hollow fiber membranes (HFMs).
- Comprehensive characterization including physico-chemical analysis, hemocompatibility testing, and cytotoxicity assays.
- Evaluation of HepG2 cell adhesion, proliferation, spheroid formation, albumin secretion, urea synthesis, and cytochrome P450 activity.
Main Results:
- The PCL-C-G NFs-deposited HFMs demonstrated superior hemocompatibility, making them suitable for blood-contact applications.
- Minimal cytotoxicity was observed for all developed matrices.
- HepG2 cells showed robust adherence, proliferation, and formed multicellular spheroids on the PCL-C-G matrix.
- Key liver-specific functions, including albumin secretion and urea synthesis, were maintained.
Conclusions:
- The developed 3D multiscale fiber-based matrix offers a promising platform for cell growth and functional maintenance.
- This matrix exhibits excellent biocompatibility and hemocompatibility, suitable for blood-contact applications.
- The matrix holds potential as a membrane for bioreactor systems and bio-artificial liver devices.
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