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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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Related Experiment Video

Updated: Feb 15, 2026

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
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RNA helicases in RNA decay.

Vanessa Khemici1, Patrick Linder2

  • 1Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.

Biochemical Society Transactions
|January 21, 2018
PubMed
Summary

RNA-degrading complexes, like bacterial degradosomes and eukaryotic exosomes, partner with RNA helicases to process difficult RNA structures. This collaboration involves specific helicase families, such as DEAD-box, Ski2-like, and Suv3, enhancing RNA degradation efficiency.

Keywords:
RNA decayRNA helicasesdegradosomeexosome

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • RNA molecules often fold into complex structures or bind to complementary RNAs, hindering their degradation by exoribonucleases.
  • Efficient RNA turnover is crucial for cellular function and requires specialized machinery to overcome these processing challenges.

Purpose of the Study:

  • To investigate the synergistic relationship between RNA-degrading complexes and RNA helicases in bacteria and eukaryotes.
  • To identify the specific types of RNA helicases associated with different RNA degradation pathways.

Main Methods:

  • Comparative analysis of RNA degradation pathways in bacterial, mitochondrial, and eukaryotic systems.
  • Identification and characterization of RNA helicase families involved in RNA processing.

Main Results:

  • Bacterial degradosomes associate with DEAD-box family RNA helicases.
  • Eukaryotic exosomes and mitochondrial degradosomes utilize Ski2-like and Suv3 RNA helicases.
  • RNA helicases facilitate the processing and degradation of structured or bound RNAs.

Conclusions:

  • RNA helicases are essential partners for RNA degradation machinery, including bacterial degradosomes and eukaryotic exosomes.
  • The specific RNA helicase families employed vary across different cellular compartments and organisms, reflecting specialized roles in RNA metabolism.