Recent trends and analytical challenges in plant bioactive peptide separation, identification and validation

Area of Science:

• Food Science
• Biochemistry
• Analytical Chemistry

Background:

• Bioactive peptides (BPs) are increasingly researched for health benefits like antioxidant and antimicrobial properties.
• Production of BPs from food by-products, especially vegetables, is a sustainable approach for value recovery.
• Vegetable sources offer a cost-effective and environmentally friendly alternative for BP extraction.

Purpose of the Study:

• To review recent advancements in the investigation of bioactive peptides from vegetable food sources and by-products.
• To discuss challenges and techniques for isolating, separating, and identifying plant-derived BPs.
• To highlight the importance of validating BP sequences and bioactivities.

Main Methods:

• Review of current literature on bioactive peptide research in vegetables.
• Discussion of chromatographic techniques for peptide isolation and separation.
• Exploration of analytical methods for peptide identification in plant matrices.

Main Results:

• Vegetable by-products are a promising source of novel bioactive peptides.
• Advanced analytical techniques are crucial for efficient BP discovery and characterization.
• Validation of peptide sequence and bioactivity is essential for application.

Conclusions:

• Bioactive peptides from vegetable sources hold significant potential for functional foods and supplements.
• Further research into efficient extraction and identification methods is warranted.
• Sustainable utilization of vegetable wastes can contribute to a circular economy.