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Updated: Feb 15, 2026

Harvesting of Peroneus Longus Tendon Autograft
Published on: September 2, 2025
ACL Reconstruction Basics: Quadruple (4-Strand) Hamstring Autograft Harvest
Rachel M Frank1, Jason T Hamamoto1, Eamon Bernardoni1
1CU Sports Medicine, Department of Orthopedics, University of Colorado School of Medicine, Boulder, Colorado, U.S.A.
This technical note details hamstring tendon autograft harvest for anterior cruciate ligament (ACL) reconstruction. It focuses on a quadruple hamstring graft technique using gracilis and semitendinosus tendons for enhanced biomechanical strength.
Area of Science:
- Orthopedic Surgery
- Sports Medicine
- Biomedical Engineering
Background:
- Hamstring tendon autografts are a common choice for anterior cruciate ligament (ACL) reconstruction.
- Advantages include reduced postoperative pain and simpler recovery compared to bone patellar tendon bone grafts.
- Quadruple hamstring grafts offer significant biomechanical strength.
Purpose of the Study:
- To describe a precise technique for harvesting hamstring autografts for ACL reconstruction.
- To detail the use of gracilis and semitendinosus tendons for a quadruple (4-strand) graft.
- To emphasize critical steps for avoiding iatrogenic neurovascular damage and graft injury.
Main Methods:
- Description of a specific surgical technique for hamstring autograft harvest.
- Utilizing both gracilis and semitendinosus tendons for graft preparation.
- Emphasis on meticulous technique during tendon stripper use.
Main Results:
- A reproducible method for obtaining a quadruple hamstring autograft is presented.
- The technique aims to minimize risks associated with graft harvest.
- Highlights the biomechanical advantages of the quadruple hamstring graft.
Conclusions:
- The described technique provides a reliable method for hamstring autograft harvest for ACL reconstruction.
- Attention to surgical detail is crucial for optimal graft integrity and patient safety.
- This approach facilitates the creation of a strong, quadruple hamstring graft for anterior cruciate ligament repair.
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