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Revolution in Genotyping Platforms for Crop Improvement.
Armin Scheben1, Jacqueline Batley1,2, David Edwards3,4
1School of Biological Sciences, University of Western Australia, Crawley, WA, Australia.
Advances in Biochemical Engineering/Biotechnology
|January 23, 2018
Summary
High-throughput sequencing advances crop genotyping using single nucleotide polymorphisms (SNPs). Genotyping by sequencing (GBS) methods are becoming more prevalent due to lower costs and increased data volume, accelerating crop improvement.
Area of Science:
- Genomics
- Plant Science
- Biotechnology
Background:
- High-throughput sequencing has revolutionized crop genotyping.
- Decreasing sequencing costs enable large-scale DNA marker analysis.
Purpose of the Study:
- To outline and compare novel crop genotyping platforms.
- To provide a perspective on the future of crop genomics.
Main Methods:
- Comparison of array technologies and genotyping by sequencing (GBS) methods.
- GBS approaches include reduced representation sequencing (RRS) and skim sequencing.
Main Results:
- SNP arrays offer high quality and straightforward analysis.
- GBS methods, particularly RRS and skim sequencing, are cost-effective and generate substantial data.
Conclusions:
- Genotyping by sequencing (GBS) is expected to become more prevalent in crop genomics.
- Streamlined data analysis and decreasing sequencing costs will drive GBS adoption for accelerated crop improvement.
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