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Related Experiment Video

Updated: Feb 15, 2026

Measuring Replicative Life Span in the Budding Yeast
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Measuring Replicative Life Span in the Budding Yeast

Published on: June 25, 2009

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Regulation of Replication Origins.

Anna B Marks1, Haiqing Fu1, Mirit I Aladjem2

  • 1Developmental Therapeutics Branch, Center for Cancer Research, NCI, NIH, Bethesda, MD, USA.

Advances in Experimental Medicine and Biology
|January 23, 2018
PubMed
Summary

Eukaryotic DNA replication uses flexible initiation sites, not all of which are active each cell cycle. This origin choice is coordinated with gene expression and chromatin structure, ensuring genomic stability.

Keywords:
Cell cycle regulationChromatin organizationDNA replicationHistone modificationReplication origin licensingReplication timing

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Eukaryotic genome duplication initiates at multiple replication origins, a process spatially and temporally regulated.
  • Unlike prokaryotes, eukaryotes utilize only a subset of potential replication origins per cell cycle.
  • Origin usage varies across cell types, correlating with DNA packaging and gene expression.

Purpose of the Study:

  • To explore the mechanisms and significance of replication origin selection in eukaryotes.
  • To understand how origin flexibility contributes to cell type-specific DNA replication and chromatin organization.
  • To investigate the role of origin features in maintaining genomic stability.

Main Methods:

  • Comparative analysis of replication origin characteristics in different eukaryotic systems (e.g., budding yeast vs. metazoans).
  • Examination of the association between replication origins and chromatin structure, histone modifications, and transcriptional activity.
  • Investigation of DNA-DNA and DNA-protein interactions at replication origins.

Main Results:

  • Budding yeast replication origins are defined by a consensus sequence.
  • Metazoan replication origins lack a consensus sequence but are characterized by structural and epigenetic features.
  • These features include chromatin packaging, histone modifications, transcription, and DNA interactions, conferring cell type-specific replication.

Conclusions:

  • Eukaryotic replication origin selection is a flexible process crucial for coordinating DNA replication with gene expression and chromatin structure.
  • Cell type-specific features at metazoan origins regulate replication timing and contribute to genomic stability.
  • Understanding origin regulation is key to comprehending genome duplication and cellular function.