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Updated: Feb 15, 2026

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
Published on: June 21, 2011
FIMic: design for ultimate 3D-integral microscopy of in-vivo biological samples
G Scrofani1, J Sola-Pikabea1, A Llavador1
1Department of Optics, University of Valencia, E-46100 Burjassot, Spain.
Abstract:
In this work, Fourier integral microscope (FIMic), an ultimate design of 3D-integral microscopy, is presented. By placing a multiplexing microlens array at the aperture stop of the microscope objective of the host microscope, FIMic shows extended depth of field and enhanced lateral resolution in comparison with regular integral microscopy. As FIMic directly produces a set of orthographic views of the 3D-micrometer-sized sample, it is suitable for real-time imaging. Following regular integral-imaging reconstruction algorithms, a 2.75-fold enhanced depth of field and [Formula: see text]-time better spatial resolution in comparison with conventional integral microscopy is reported. Our claims are supported by theoretical analysis and experimental images of a resolution test target, cotton fibers, and in-vivo 3D-imaging of biological specimens.
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